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Laboratory Techniques with Reagents and Solutions
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Chavan, U D
2018, viii+400p., figs., tbls., 25cm
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Weight :  0 gms
Binding :  Hard Bound
Height :  25 cm
Width :  15 cm
Imprint :  Daya Publishing House
Year :  2018
 

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ISBN: 9789351248439
Status: In Stock 
Price: Rs 2995.00  

This book contains most suitable procedure of laboratory techniques. It describes the basic laboratory rules, equipment and instrumentation, basic concepts, types of stock standard solutions, equivalent concentration, normality, normal solution, molar solution, molal solution, percent solution, parts per million (ppm), various types of buffer solutions, pH, spectrophotometry, atomic absorption spectrophotometry, chromatography viz. paper chromatography, thin layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC), gas-liquid chromatography (GLC), high performance liquid chromatography (HPLC), polymerase chain reaction (PCR), scanning electron microscope (SEM), radiation, enzyme assays and radioactivity and related calculations. These equipment and instruments are commonly used in practical laboratory. Important references have been also cited for detailed reading. It is hoped that the book is quite useful for students, teachers, researchers, biochemists, chemist, food chemist and person engaged in various laboratories.

Preface v
1. Introduction
Basic Laboratory Rules
Basic Equipment and Instrumentation 1
2. Some Basic Concepts
Scientific Notation
Significant Figures
Recording of Results
Expressing Concentration of Solute
Converting between Concentration Units
Dilution of Concentration Solutions 41
3. Types of Stock Standard Solutions
Introduction
Ionic Standard Solution
Element Standard Solution
Formula Standard Solution 55
4. Equivalent Concentration
Normal solutions
Molar/Molarity solutions
Molal solutions
Percent solutions
Parts Per Million (PPM Solution) 65
5. Buffer Solutions
Principles
Preparation
Calculations 127
6. pH
Acid and Bases
Hydrogen ion Concentration and pH
Measurement of pH 159
7. (i). Spectrophotometry
Basic Principles
Quantitative Applications
(ii). Atomic Absorption Spectrophotometry
Basic Principles
Quantitative Applications 165
8. Chromatography
Principles and Applications
Paper Chromatography
Thin Layer Chromatography (TLC)
Gas Chromatography (GC)
Liquid Chromatography (LC)
Gas-Liquid Chromatography (GLC)
High Performance Liquid Chromatography (HPLC)
Polymerase Chain Reactions (PCR)
Scanning Electron Microscope (SEM)
Radiation 247
9. Enzyme Assays
Principles and Applications
Photometric
Radiometric
Electrochemical Assays
Gel Electrophoresis 323
10. Radioactivity and related calculations 363
References 381
Appendices 399

Dr. U.D. Chavan obtained his M.Sc. (Ag) Biochemistry degree from Mahatma Phule Krishi Vidyapeeth, Rahuri in 1987. He received his Ph.D. degree in Food Science from Memorial University of Newfoundland
St. John’s Canada in 1999. He has done International training on “Global Nutrition 2002” at Uppsala University Uppsala, Sweden in 2002. He also attended follow-up International workshop on “Global Nutrition 2002” at Hanoi, Vietnam in 2002. Dr. Chavan visited Denmark, Finland, Ireland, France, Switzerland, Poland, Spain, Vietnam, Thailand, England, and U.S.A. under “Global Nutrition 2002” Programme. Dr. Chavan worked as Senior Research Assistant in the Department of Biochemistry and Food Science and Technology at Mahatma Phule Krishi Vidyapeeth Rahuri from 1988 to 2000. During his Ph.D., he worked as Technician/Research Associate at Atlantic Cool Climate Crop Research Center and Agriculture and Agri-Food Canada. He received D.Sc. degree in 2006 from USA. He has guided 22 students for M.Sc. (Agri.) in Biochemistry and Food Science and Technology. From 2000 to 2004 he worked as an Assistant Professor of Biochemistry at Mahatma Phule Krishi Vidyapeeth Rahuri. Now, he is working as a Professor in the Department of Food Science and Technology, Senior Cereal Food Technologist in Sorghum Improvement Project and Foreign Student Advisor at Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist, Ahmednagar, Maharashtra, India.

   

  Microbiology
Biochemistry
Biotechnology
Food Science
Lab Manual
 
   

   
   

  U. D. Chavan
 
   

  TLC,
HPLC,
PCR,
PPM,
Buffer
Solution,
PPB,
Atomic
Unit,
Enzyme
Assay,
Sports,
Anti-doping
analys
 
   
 
 
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Laboratory Techniques with Reagents and Solutions

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