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Biological Radiation Protection: A Protocol Manual
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Raj Kumar
xxii+244 p., 25 cm
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Weight :  558 gms
Binding :  Hard Bound
Height :  25 cm
Width :  15 cm
Imprint :  Daya Publishing House
Year :  2017
 

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ISBN: 9789351247869
Status: In Stock 
Price: Rs 1595.00  

This experimental manual provides comprehensive information about the designing and execution of the experimental protocols for radioprotective drug screening. The manual divided into seven sections. Each section arrange in chronological order from preliminary to advance methods to evaluate a drug molecule for its probable radioprotective properties. This manual is first of its kind in the area of radiation biology and radioprotection. Preliminary in vitro assay (section I) designed to evaluate in vitro antioxidant activity of test compound which are integral part of radioprotection. Secondary methods (Section II) designed to evaluate radioprotective efficacy of test compound towards biomacromolecules i.e. proteins, enzymes and plasmid DNA. The third section (Section III) of this manual dedicated to evaluate in vitro radioprotective potential of test compound in cell culture in terms of colony forming unit (CFU), superoxide dismutase, catalase, glutathione reductase, glutathione-s-transferase, and lysosomal membrane stability. Radiation induced gemonic DNA damage and apoptosis is the common cause of cell death in cells. Therefore, to assess DNA damage and apoptosis inhibitory activity of the test compound a complete section (section IV) was formulated. Further, to estimate mitochondrial DNA damage and perturbation in oxidative phosphorylation as well its modulation by radioprotective drug molecule detailed protocols were mentioned in section V and section VI of the manual. Last but not the least, section VII of this manual was designed to evaluate immune system radioprotection by radioprotective compound. The manual arranged in systematic manner, by follow the protocols of this manual researcher can confirm and classify the radioprotective efficacy of the test molecule.

Foreword I- Dr. A.K. Singh, Director, INMAS, Delhi vii
Foreword II- Dr. Rakesh Kumar Sharma, Director, DFRL, Mysore ix
Foreword III- Dr. Manju Gupta, Addl. Director, INMAS, Delhi xi
Preface xiii
Section I
Preliminary In vitro Methods to Screen Out Radioprotective Compounds
Section II
Secondary Methods to Screen Out Radioprotective Compounds
Section III
Advance In vitro Methods of Radioprotective Efficacy Evaluation
Section IV
Determination of Radiation Induced Apoptosis and DNA Damage
Section V
Radiation Induced Mitochondrial DNA Damage and its Modulation by Radioprotective Drug Treatment
Section VI
Radiation Induced Mitochondrial Oxidative Phosphorylation Perturbations and their Modulation by Radioprotective
Compounds Treatment
Section VII
Immune System Radioprotective Efficacy Evaluation of
Radioprotective Compounds

Index 237

Dr. Raj Kumar, Scientist-D and Head Radiation Biotechnology group, Division of Radioprotective Drug Development and Research (DRDDR), Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research and Development Organization (DRDO), Delhi did his post-graduate in Microbiology from Gurukul Kangri University Haridwar in 1994. Dr. Raj Kumar completed his Ph.D. in Biotechnology from Indian Institute of Technology Roorkee (IIT-Roorkee) in 2001. In the same year he joined INMAS, DRDO, Ministry of Defence, Govt of India. Till date he has published more than 60 research papers in International Scientific Journals of repute in the area of radioprotection. He has also contributed 7 book chapters in different books published by reputed International publishers.

   

  Biochemistry
Biotechnology
Chemistry
Environmental Science
Public Health
 
   

   
   

  Raj Kumar
 
   

  Radioprotective
Compounds,
Antiradical
Power
(CARP),
Gamma
Radiation
Oxygen
Radical
Absorbance
Capa
 
   
 
 
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Biological Radiation Protection: A Protocol Manual

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