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Plant Genetic Transformation Technology
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Wagdy A. Sawahel
138p., 23 cm
First Published, 1997
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Weight :  300 gms
Binding :  Hard Bound
Height :  23 cm
Width :  15 cm
Imprint :  Daya Publishing House
Year :  2017
 

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ISBN: 9788170351757
Status: In Stock 
Price: Rs 895.00  

The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology.

Contents Chapter 1: Genetic Transformation
History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability
Chapter 2: Gene Delivery systems
Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication
Chapter 3: Strategies for Improving Transformation Efficiency
Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence
Chapter 4: Organelle Transformation
Chapter 5: Shotgun Transformation
Plasmid rescue, Gene rescue, Promoter & enhancer rescue.

   

  Genetics And Plant Breeding
Biotechnology
 
   

   
   

  Wagdy A. Sawahel
 
   

   
   
 
 
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Plant Genetic Transformation Technology

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